statistics toolbox release 2016 b Search Results


incucyte zoom version 2016b  (Sartorius AG)
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Sartorius AG incucyte zoom version 2016b
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program written in matlab 2016b  (MathWorks Inc)
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MathWorks Inc program written in matlab 2016b
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2016b  (MathWorks Inc)
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MathWorks Inc 2016b
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scils lab software  (Bruker Corporation)
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Bruker Corporation scils lab software
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b thetaiotaomicron  (DSMZ)
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DSMZ b thetaiotaomicron
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
B Thetaiotaomicron, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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johansen & esbaugh 2017  (Mager Scientific)
90
Mager Scientific johansen & esbaugh 2017
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
Johansen & Esbaugh 2017, supplied by Mager Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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algorithms matlab  (MathWorks Inc)
96
MathWorks Inc algorithms matlab
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
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scils lab version 2016b  (Bruker Corporation)
90
Bruker Corporation scils lab version 2016b
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
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b saxobsidens bc444t  (DSMZ)
86
DSMZ b saxobsidens bc444t
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
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statistics toolbox release 2016b  (MathWorks Inc)
90
MathWorks Inc statistics toolbox release 2016b
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
Statistics Toolbox Release 2016b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plastic eppendorf tubes  (Eppendorf AG)
90
Eppendorf AG plastic eppendorf tubes
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
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version 2016 b  (MathWorks Inc)
90
MathWorks Inc version 2016 b
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
Version 2016 B, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. thetaiotaomicron . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).

Journal: Nature Communications

Article Title: Sialic acid catabolism drives intestinal inflammation and microbial dysbiosis in mice

doi: 10.1038/ncomms9141

Figure Lengend Snippet: ( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. thetaiotaomicron . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).

Article Snippet: E. coli (EHV2) and B. thetaiotaomicron (DSMZ 2079T) were fixed in 0.5% paraformaldehyde for 15 min at room temperature and washed with PBS before stimulation.

Techniques: Bacteria, Cell Culture, Expressing, Flow Cytometry, Fluorescence, Comparison, Control